TABLE 4.
Susceptibility patterns of S. aureus RN4220 strains diploid for grlB+ and mutant grlB
| Strain/plasmid | Type of grlBa in:
|
MIC (μg/ml)b of:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Chromosome | Plasmid | CPFX | NFLX | OFLX | SPFX | NA | OA | NOV | COU | |
| RN4220/pSK950 | Wild | None | 0.5 | 1.0 | 0.5 | 0.06 | 64.0 | 2.0 | 0.16 | 0.01 |
| RN4220/pBFC9B | Wild | N470D | 1.0 | 8.0 | 1.0 | 0.12 | 32.0 | 2.0 | 0.04 | 0.0025 |
| EN22/pSK950 | N470D | None | 1.0 | 8.0 | 1.0 | 0.12 | 64.0 | 2.0 | 0.04 | 0.0025 |
| EN22/pBFISB | N470D | Wild | 0.25 | 0.5 | 0.25 | 0.06 | 64.0 | 2.0 | 0.08 | 0.01 |
a
Wild, wild-type gene; none, plasmid vector only; N470D, mutant gene (grlB543) encoding Asn470Asp mutation.
b
CPFX, ciprofloxacin; NFLX; norfloxacin; OFLX, ofloxacin; SPFX, sparfloxacin; NA, nalidixic acid; OA, oxolinic acid; NOV, novobiocin; COU, coumermycin. The MICs are the means of three different transformants.