TABLE 5.
Susceptibility patterns of S. aureus MT5 gyrB142 strains diploid for grlB+ and mutant grlBa
| Strain/plasmid | Type of grlBb in:
|
Plasmid positionc | MIC (μg/ml)d
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chromosome | Plasmid | CPFX | NFLX | OFLX | SPFX | NA | OA | NOV | COU | ||
| MT5/pSK950 | Wild | None | Plasmid | 0.125 | 0.5 | 0.25 | 0.06 | 128.0 | 4.0 | 40.0 | 5.0 |
| MT5/pBFC9B | Wild | N470D | Plasmid | 1.0 | 4.0 | 1.0 | 0.12 | 128.0 | 2.0 | 10.0 | 1.25 |
| MT5224c9/pSK950 | N470D | None | Plasmid | 1.0 | 8.0 | 1.0 | 0.12 | 128.0 | 4.0 | 5.0 | 1.25 |
| MT5224c9/pBFISB | N470D | Wild | Plasmid | 0.125 | 0.25 | 0.25 | 0.06 | 128.0 | 4.0 | 40.0 | 5.0 |
| MT5/pSK950 | Wild | None | Chromo. | 0.125 | 0.25 | 0.25 | NDe | ND | ND | 40.0 | 5.0 |
| MT5/pBFC9B | Wild | N470D | Chromo. | 0.5 | 1.0 | 0.5 | ND | ND | ND | 20.0 | 5.0 |
| MT5224c9/pSK950 | N470D | None | Chromo. | 1.0 | 4.0 | 1.0 | ND | ND | ND | 2.5 | 0.62 |
| MT5224c9/pBFISB | N470D | Wild | Chromo. | 0.25 | 1.0 | 0.5 | ND | ND | ND | 40.0 | 5.0 |
a
MT5 and MT5224c9 carry a double mutation of gyrB (Ile102Ser and Arg144Ile).
b
Wild, wild-type gene; none, plasmid vector only; N470D, mutant gene (grlB543) encoding Asn470Asp mutation.
c
Position of the plasmid: free in the cell (plasmid) or integrated in the chromosome (chromo.).
d
CPFX, ciprofloxacin; NFLX; norfloxacin; OFLX, ofloxacin; SPFX, sparfloxacin; NA, nalidixic acid; OA, oxolinic acid; NOV, novobiocin; COU, coumermycin. The MICs are the means of three different transformants.
e
ND, not determined.