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. 2023 Oct 3;6:0247. doi: 10.34133/research.0247

Fig. 1.

Fig. 1.

PLMDs plus RT enhanced DNA damage and induced ICD markers in vitro. (A) Immunofluorescence images of γ-H2AX staining of TRAMP-C2 cells 4 and 24 h after treatment with saline, 12.5 μM PLMDs, RT (6 Gy), and PLMDs + RT under hypoxic conditions. Scale bars, 50 μm. (B) HMGB1 release and (C) ATP secretion in hypoxic PC3, DU145, and TRAMP-C2 cells in vitro. (D) Representative CLSM images of CRT exposure in hypoxic PC3 and TRAMP-C2 cells in vitro. Scale bars, 20 μm. PCa cells were treated with saline, PLMDs (12.5 μM), RT (6 Gy), or PLMDs plus RT under hypoxic conditions. HMGB1 release and ATP release were assessed 24 h post-treatment, and CRT exposure was assessed 4 h post-treatment. Results mean +SD for ≥ 3 independent experiments. P < 0.05 is considered statistically significant. P < 0.05 (*), P < 0.005 (**), P < 0.0005 (***), and P < 0.0001 (****). DAPI, 4′,6-diamidino-2-phenylindole.