Table 2.

Association of ESR1 and PIK3CA codon variants and alterations in oncogenic pathways with overall survival (OS).

	HR	95% C.I		P value
ESR1 variants
Wild type	1
537	1.08	0.68	1.71	0.7594
538	0.77	0.46	1.32	0.3446
380	2.48	1.01	6.07	0.0476
536	1.06	0.41	2.74	0.9071
PIK3CA variants
Wild type	1
1047	0.97	0.6	1.55	0.8871
545	0.61	0.34	1.08	0.089
542	1.12	0.65	1.91	0.6849
RAS pathway SNVs
Not altered	1
Altered	1.96	1.16	3.33	0.0126
P53 pathway SNVs
Not altered	1
Altered	1.38	1.02	1.86	0.0341
Cell cycle pathway SNVs
Not altered	1
Altered	1.07	0.53	2.16	0.8576
ER pathway SNVs
Not altered	1
Altered	1.41	0.84	2.36	0.1948
PI3K pathway SNVs
Not altered	1
Altered	1.57	0.93	2.66	0.0925
RTK pathway CNVs
Not altered	1
Altered	1.2	0.77	1.86	0.4269
RAS pathway CNVs
Not altered	1
Altered	0.84	0.34	2.08	0.7009
RAF pathway CNVs
Not altered	1
Altered	0.91	0.32	2.55	0.8563
ER pathway CNVs
Not altered	1
Altered	0.93	0.23	3.83	0.9222
MYC pathway CNVs
Not altered	1
Altered	1.91	1.08	3.38	0.027
Cell cycle pathway CNVs
Not altered	1
Altered	1.27	0.79	2.05	0.3275
PI3K pathway CNVs
Not altered	1
Altered	2.27	1.16	4.47	0.0174
Lung involvement
No	1
Yes	1.28	0.96	1.73	0.0961
Liver involvement
No	1
Yes	1.84	1.36	2.5	0.0001
Bone involvement
No	1
Yes	1.66	1.13	2.43	0.0093
Soft tissue involvement
No	1
Yes	2.01	1.41	2.85	0.0001
Previous CDK4/6i
No	1
Yes	1.14	0.82	1.58	0.4464
Treatment line
First	1
Second	2.24	1.39	3.63	0.001
Third	3.58	2.16	5.94	< 0.0001
Fourth	2.44	1.34	4.43	0.0035
Fifth and beyond	3.52	2.18	5.68	< 0.0001

CNVs, Copy Number Variations; SNVs, Single Nucleotide Variations