TABLE 4.

Percent inhibition by DCQAs of HIV-1 RNase H-catalyzed RNA-DNA hybrid cleavage

Compound	No preincubationa			Preincubationb
	Expt 1	Expt 2	Avg
Quinic acid	7	18	13	17
Caffeic acid	6	2	4	1
Chlorogenic acid	−9	9	0	4
1-MO-3,5-DCQA	37	18	28	41
1,5-DCQA	−7	−15	−11	5
4,5-DCQA	−27	−19	−23	12
3,5-DCQA	−29	−24	−26	−11
3,4-DCQA (synthetic)	−26	−22	−24	−10
l-CCA	14	−5	5	22

^aCompounds were tested with the Amersham HIV-1 IN SPA kit according to the manufacturer’s instructions. Values are percent inhibitions of reactions after 15 min. Compounds were added to the reaction mix at the same time as the full-length RT proteins. The final concentration of each compound was 50 μg/ml. Values for each experiment are means of three separate reactions. 

^bCompounds were preincubated with HIV-1 RT for 1 h at room temperature prior to testing in the Amersham HIV-1 RNase H SPA kit. Values are percent inhibitions of reactions after 15 min. The final concentration of each compound was 50 μg/ml. Results are means of triplicate samples in one experiment.