figure 5.

pdhc does not compromise oc’s coupling to obs. for (a, b), mscs and obs (14d-osteogenically-differentiated mscs) were treated with the conditioned medium harvested from pdhc disk alone (disk group), ocs (oc group), or pdhc-modified ocs (ocdisk group). a control group without any treatment was also included (nt group). (a) cell viability determined by mts absorbance. (b) dapi staining results of 16h transwell cell migration assay. for (c, d), indicated conditioned medium, with or without osteogenic medium (om), was used for osteogenic induction of mscs. (c) relative expression of osteogenic genes on day 7, 14, 21, and 28 determined by rt-qpcr; normalized to b2m. (d) mineral nodule visualization and quantification on day 28 by alizarin red staining.

n = 3 for all experiments. *p < 0.05, ns: p > 0.05. data shown as mean ± sem.