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. 2023 Oct 3;18(10):e0287817. doi: 10.1371/journal.pone.0287817

Fig 2. Effects of Tβ4 on MDA, SOD, LDH, GSH-PXand ROS in Aβ-treated SH-SY5Y cells.

Fig 2

SH-SY5Y cells transduced with Lentivirus empty vector or Lentivirus-Tβ4 were treated with Aβ at the concentration of 25 μM Aβ, or left untreated for 48 hours. The levels of MDA (A), SOD (B), LDH (C), and GSH-PX (D) in SH-SY5Y cells were analyzed by using commercial kits. (E) Cells were stained with DHE, observed under a fluorescence microscope, detected by a fluorescence microplate reader and expressed as fold of non-treated normal group (F). (G) The fluorescence intensity in different cells was analyzed by flow cytometry. The data are shown as mean ± SD of three independent experiments each in triplicate. Scale bars, 100 μm. NS, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.