Fig 4. Effects of Tβ4 on ERK/p38 MAPK signaling in Aβ-treated SH-SY5Y cells.

SH-SY5Y cells with or without overexpression of Tβ4 were treated with Aβ at a concentration of 25 μM for 48 h prior to western blot analysis to measure the levels of (A) p-p38/p38, (B) p-JNK/JNK and (C) p-ERK/ERK. β-actin was used as an internal control. The bars represent the mean ± SD in the different experimental groups (n = 3). NS, not significant; **, p < 0.01; ***, p < 0.001.