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. 2023 Oct 3;18(10):e0287817. doi: 10.1371/journal.pone.0287817

Fig 6. Tβ4 inhibits ERK activation through up-regulating 5-HTR1A in Aβ-treated SH-SY5Y cells.

Fig 6

(A) SH-SY5Y cells were transfected with 5-HTR1A siRNAs for 48 h before the mRNA level of 5-HTR1A was quantified by qPCR. (B and C) Lentivirus-T4β transduced SH-SY5Y cells were transfected with 5-HTR1A siRNA and subsequently subjected to Aβ treatment at 25 μM for 48 h prior to western blot analysis to measure the expression of JNK/pJNK, p38/pp38 and ERK/pERK. β-actin was used as an internal control. The bars represent the mean ± SD in the different experimental groups (n = 3). NS, not significant; **, p < 0.01; ***, p < 0.001.