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. 2023 Aug 29;12(17):e028185. doi: 10.1161/JAHA.122.028185

Figure 5. TRAF4 overexpression promotes the activation of AKT signaling pathway.

Figure 5

A, WB analysis of protein levels of total and p‐AKT (phosphorylated protein kinase B) pathway‐related proteins (p‐AKT, p‐GSK3β [phosphorylated glycogen synthase kinase 3 beta], p‐mTOR [phosphorylated mammalian target of rapamycin], p‐P70S6K [phosphorylated ribosomal protein S6 kinase], AKT, GSK3β, mTOR, and P70S6K) in left ventricular tissues from wild‐type (WT) and tumor necrosis factor receptor–associated factor 4 (Traf4) knock‐out (KO) mice at 4 weeks after transverse aortic constriction (TAC) surgery; GAPDH served as a loading control. B, Protein levels of total and phosphorylated AKT pathway‐related proteins in adenovirus vector (AdVector)‐ and AdTraf4‐infected NRCMs treated with phenylephrine (PE) for 24 h. Statistical analysis was carried out by 2‐tailed Student t test. **P<0.01 vs WT TAC or AdVector PE. NRCMs indicates neonatal rat cardiomyocytes; TRAF4, tumor necrosis factor receptor–associated factor 4; and WB, Western blotting.