Fig. 7.

Blocking proteasome activity reverses TAC-induced cardiac dysfunction and hypertrophic remodeling. a, WT and ATGL KO mice were subjected to TAC operation and co-treated with VO-OHpic (10 mg/kg daily) or epoxomicin (2.9 mg/kg daily) continuously for 4 weeks. Echocardiographic measurement of LV chamber (left), and the calculation of LV FS% (right, n = 6). b, Representative H&E staining of heart sections (left). Scale bar: 10 mm. The ratio of HW/TL (right, n = 6). c, TRITC-labeled WGA staining of heat sections (left). Quantification of cross-sectional area of myocytes (right, n = 6, 150–200 cells counted per sample). Scale bar: 50 μm. d, Masson’s trichrome staining of heat sections (left). Quantification of fibrotic area (right, n = 6). Scale bar: 50 μm. e, DHE staining of heart sections (left). Quantification of the relative superoxide production (right, n = 6). Scale bar: 50 μm. f, TUNEL staining (left). Quantification of the TUNEL-positive nuclei (right, n = 6). Scale bar: 50 μm. Data are presented as mean ± SEM, and n represents number of animals