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. 2023 Sep 20;11:1122408. doi: 10.3389/fcell.2023.1122408

FIGURE 1.

FIGURE 1

LPS induces the expression and secretion of IL-8 in cardiac fibroblasts (CF). (A) CF were stimulated with LPS (1 μg/ml) for 4, 8, and 24 h mRNA of IL-8 was analyzed by RT-qPCR. (B) CF were stimulated with LPS (1 μg/ml) at 8, 24 or 48 h. Secreted IL-8 levels were quantified using an ELISA kit. (C) CF were pre-stimulated with the TLR4 inhibitor TAK-242 (4 uM) and subsequently stimulated with LPS (1 μg/ml) for 8 h. IL-8 mRNA was determined with an RT-qPCR. (D) IL-8 secretion via TLR4 in CF. CF were pre-stimulated with the TLR4 inhibitor TAK-242 (4 uM) and subsequently stimulated with LPS (1 μg/ml) for 24 h. IL-8 secretion was determined with an ELISA kit. VEH: DMSO (vehicle for TAK-242). ***p < 0.001; **p < 0.01; *p < 0.05 vs. control; #p < 0.05 vs. LPS (-). Results are expressed as mean ± SEM. (n = 3).