FIGURE 5.

The IFN-β/JAK signaling pathway prevents LPS-induced neutrophil adhesion. Representative diagrams of adhesion of neutrophils to CF pre-stimulated with IFN-β (500 IU/mL) for 1 h and/or LPS (LPS (1 μg/ml) for 24 h in the absence of ruxolitinib ((A): cardiac fibroblast; (B) neutrophils and (C) other leukocytes and cell death). CF with adherent leukocytes were trypsinized for their removal from the plate and then were incubated with the anti RP-1(PE) antibody, specific for neutrophils, for 30 min. Adhesion levels were determined by flow cytometry. The graph shows adhesion levels of neutrophils to a CF monolayer treated as observed in the graph. The percentage of neutrophil adhesion was calculated as follows: 100 x (n° cells RP-1⁺ adhered/n° total cells RP-1⁺). **p < 0.01 vs. control; ##p < 0.01 vs. LPS; &&p < 0.01 vs. LPS + IFN-β. Results are expressed as mean ± SEM (n = 3).