Figure 5.

Hydrogels promoted the polarization of M2 macrophage in the diabetic wound beds. (A) Schematic showing that platelets promote M2 macrophage polarization by releasing growth factors through degranulation. (B) IHC staining of TGF-β1. (C) The Semi-quantification of IHC staining of TGF-β1 (n = 3). (D) IHC staining of VEGF. (E) The Semi-quantification of IHC staining of VEGF (n = 3). (F) Representative images of F4/80 (green) and CD206 (red) immunostaining showed accumulation of M2 macrophages at the wound bed on Days 6 and 15. (G) IF staining analysis of M2 polarization. Data are reported as mean ± SD (∗ indicates significant differences between control and other groups, # indicates significant differences between the two groups connected by the line segment.) ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.