Figure 6.

DPLG increased HIF-1α expression and angiogenesis. (A) Schematic showing that DFO promotes angiogenesis in conjunction with VEGF released from platelet degranulation. (B) IHC staining of HIF-1α. (C) Semi-quantification of IHC staining of HIF-1α (n = 3). (D) IHC staining of CD31. (E) Semi-quantitative analysis of total blood vessels by IHC staining of CD31 (n = 3). (F) IF co-staining for α-SMA and CD31 (∗ indicates significant differences between control and other groups, # indicates significant differences between the two groups connected by the line segment.) Data are reported as mean ± SD. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.