Furrow ingression of cyk-1(RNAi);septinunc-61(RNAi) embryos requires ANI-1
(A) During cytokinesis, CYK-1 polymerizes the unbranched F-actin of the contractile ring. In cyk-1ts mutants, no contractile ring forms and cytokinesis fails, but, surprisingly, furrow ingression was restored by depleting septin.79
(B) Central plane images of embryos expressing a membrane and DNA marker, treated with the indicated RNAi conditions. Time is indicated in seconds (s) after NEBD. Scale bar, 5 μm.
(C) The contractile ring diameter is plotted over time for individual embryos for indicated RNAi conditions. Green and red encircled stars indicate whether embryos succeed or fail cytokinesis, respectively. n = number of embryos.