Figure 1. MARCH5 is required for the in vivo expression of IL‐1β and IL‐18 and lethality in response to bacterial infection.

• A, B
  March5 cKO mice are resistant to P. aeruginosa infection (A) survival rates (n = 8) and (B) changes in the body weight of March5 ^fl/fl and March5 ^{fl/fl;Lyz‐Cre} (n = 8) mice after intraperitoneal injection with 1 × 10⁷ CFU Pseudomonas aeruginosa.
• C–F
  TNF‐α (C), IL‐6 (D), IL‐1β (E) and IL‐18 (F) from serum, spleen homogenate and peritoneal fluid collected from mice (n = 8) that were sacrificed 12 and 24 h following the bacterial infection. Each cytokine was analyzed by ELISA.

Data information: Values, *P < 0.05, **P < 0.01 (two‐tailed Student's t‐test or Mantel–Cox test). Data were expressed as the mean ± SEM. See also Fig EV2.

Source data are available online for this figure.