Figure EV1. Generation of March5 ^fl/fl and March5 ^{fl/fl; Lyz‐Cre} mice.

• A
  A schematic drawing of the March5 locus and targeting vector. C57BL/6 March5 ^tm1a mice harboring the FRT and Lox P sites. generates floxed March5 mice, Flp recombinase targets FRT sites (March5 ^tm1c ). For MARCH5 gene deletion, Cre recombinase targets Lox P sites (March5 ^tm1d ).
• B
  PCR analysis to confirm the deletion of MARCH5 exon 3 by targeted Lysozyme‐Cre recombinase. Arrows indicate the sites recognized by the primers. Total mRNA was extracted from the indicated organs from March5 ^fl/fl and March5 ^{fl/fl;Lyz‐Cre} mice.
• C
  Bone marrow cells were collected from March5 ^fl/fl and March5 ^{fl/fl;Lyz‐Cre} mice and differentiated for 6 days. Western blot analysis of MARCH5 protein expression. Tubulin was used as a loading control.

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