| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| HEK293T | ATCC | Cat# ACS‐4500 |
| THP‐1 | ATCC | Cat# TIB‐202 |
| L929 | Je‐Wook Yoo | N/A |
| iBMDM | Je‐Wook Yoo | N/A |
| iBMDM NLRP3‐GFP | Je‐Wook Yoo | N/A |
| Citrobacter rodentium | ATCC | Cat# 51459 |
| Salmonella typhimurium | ATCC | Cat# 14028 |
| Pseudomonas aeruginosa | ATCC | Cat# BAA‐1744 |
| March5 fl/fl mice | This paper | N/A |
| March5 fl/fl;Lyz‐cre mice | This paper | N/A |
| Recombinant DNA | ||
| pcDNA3.1 | Invitrogen Thermo Fisher | Cat# V79020 |
| pcDNA3.1‐myc‐MARCH5 | This paper | N/A |
| pcDNA3.1‐myc‐MARCH5 (2KR) | This paper | N/A |
| pcDNA3.1‐myc‐MARCH5 (H43W) | This paper | N/A |
| pcDNA6‐SFB‐MARCH5 WT | This paper | N/A |
| pcDNA6‐SFB‐MARCH5 (2KR) | This paper | N/A |
| pCMV6‐AC‐cMyc‐DDK | This paper | N/A |
| pCMV6‐NLRP3 WT‐MycDDK | This paper | N/A |
| pCMV6‐NLRP3 K93R‐MycDDK | This paper | N/A |
| pCMV6‐NLRP3 K192R‐MycDDK | This paper | N/A |
| pCMV6‐NLRP3 K194R‐MycDDK | This paper | N/A |
| pCMV6‐NLRP3 K324R‐MycDDK | This paper | N/A |
| pCMV6‐NLRP3 K430R‐MycDDK | This paper | N/A |
| pCMV6‐NLRP3 K689R‐MycDDK | This paper | N/A |
| pCMV6‐NLRP3 K696R‐MycDDK | This paper | N/A |
| pCMV6‐NLRP3 S194A‐MycDDK | This paper | N/A |
| pcDNA3.1/Myc‐NLRP3 WT | Ren et al 2019 | N/A |
| pcDNA3.1/Myc‐NLRP3 ▵PYD | Ren et al 2019 | N/A |
| pcDNA3.1/Myc‐NLRP3 ▵Linker | Ren et al 2019 | N/A |
| pcDNA3.1/Myc‐NLRP3 ▵NACHT | Ren et al 2019 | N/A |
| pcDNA3.1/Myc‐NLRP3 ▵LRR | Ren et al 2019 | N/A |
| pCMV3‐C‐FLAG‐IL‐1β | Sino Biological | Cat# HG10139‐CF |
| pCMV3‐C‐HA‐NEK7 | Sino Biological | Cat# HG11534‐CY |
| Antibodies | ||
| Mouse monoclonal anti‐NLRP3 (Cryo2‐) |
Adipogen 1:5,000 |
Cat# AG‐20B‐0014 |
| Rabbit polyclonal anti‐NLRP3 |
Cell Signaling Technology 1:1,000 |
Cat# 15101 |
| Goat polyclonal anti‐NLRP3 |
Abcam 1:1,000 |
Cat# ab4207 |
| Rabbit polyclonal anti‐ASC |
Adipogen 1:1,000 |
Cat# AG‐25B‐0006 |
| Mouse monoclonal anti‐ASC |
Santa Cruz Biotechnology 1:1,000 |
Cat# sc‐514414 |
| Rabbit polyclonal anti‐ASC |
Santa Cruz Biotechnology 1:1,000 |
Cat# sc‐22514‐R |
| Mouse monoclonal anti‐IL‐1β |
Cell Signaling Technology 1:1,000 |
Cat# 12242 |
| Goat Polyclonal anti‐IL‐1β (p20) |
Adipogen 1:1,000 |
Cat# AF‐401‐NA |
| Mouse monoclonal anti‐IL‐1β |
Santa Cruz Biotechnology 1:1,000 |
Cat# 12742 |
| Mouse monoclonal anti‐mouse caspase‐1 |
Adipogen 1:1,000 |
Cat# AG‐20B‐0042 |
| Rabbit polyclonal anti‐human caspase‐1 |
Cell Signaling Technology 1:1,000 |
Cat# 3866 |
| Mouse monoclonal anti‐human caspase‐1 |
Santa Cruz Biotechnology 1:1,000 |
Cat# sc‐56036 |
| Mouse monoclonal anti‐mouse MAVS |
Cell Signaling Technology 1:1,000 |
Cat# 83000 |
| Rabbit polyclonal anti‐human MAVS |
Cell Signaling Technology 1:1,000 |
Cat# 3993 |
| Rabbit polyclonal anti‐MARCH5 |
Shigeru YANAGI 1:1,000 |
N/A |
| Mouse monoclonal anti‐NEK7 |
Santa Cruz Biotechnology 1:1,000 |
Cat# sc‐393539 |
| Rabbit monoclonal anti‐NEK7 |
Abcam 1:1,000 |
Cat# ab133514 |
| Mouse monoclonal anti‐FLAG |
Sigma‐Aldrich 1:1,000 |
Cat# F1804 |
| Mouse monoclonal anti‐c‐Myc |
Santa Cruz Biotechnology 1:1,000 |
Cat# sc‐40 |
| Mouse monoclonal anti‐HA |
Santa Cruz Biotechnology 1:1,000 |
Cat# sc‐7392 |
| Mouse monocclonal anti‐Tubulin |
Santa Cruz Biotechnology 1:1,000 |
Cat# sc‐73242 |
| Rabbit polyclonal anti‐GAPDH |
Cell Signaling Technology 1:1,000 |
Cat# 5174 |
| Goat anti Mouse IgG (H+L)‐HRP conjugate |
Bio‐rad 1:5,000 |
Cat# 170516 |
| Goat anti Rabbit IgG (H+L)‐HRP conjugate |
Bio‐rad 1:5,000 |
Cat# 1706515 |
| Donkey anti Goat IgG (H+L)‐HRP conjugate |
Bio‐rad 1:5,000 |
Cat# 642005 |
| Oligonucleotides and other sequence‐based reagents | ||
|
Human MARCH5 targeted siRNA 5′‐GG GUG GAA UUG CGU UUG UU‐3′ |
Bioneer | This paper |
|
Mouse March5 targeted siRNA 5′‐GGU UGU AGG CCA UAA AGA A‐3′ |
Bioneer | This paper |
|
Human MAVS targeted siRNA 5′‐CAG GUU GGC CUC AUG AGA U‐3′ |
Bioneer | Cat# 57506 |
|
Mouse Asc targeted siRNA 5′‐GCU CUU CAG UUU CAC ACC A‐3′ |
Bioneer | This paper |
| Chemicals, Enzymes and other reagents | ||
| Lipopolysaccharides from Escherichia coli O26:B6 | Sigma‐Aldrich | Cat# L8274 |
| ATP | Sigma‐Aldrich | Cat# A6419 |
| Nigericin | Invivogen | Cat# tlrl‐nig |
| FLA‐ST | Invivogen | Cat# tlrl‐stfla |
| Poly(I:C) | Invivogen | Cat# tlrl‐pic |
| Poly(dA:dT) | Invivogen | Cat# tlrl‐patn‐1 |
| Recombinant Human MARCH5 GST Protein | Abnova | Cat# H00054708‐P0 |
| Anti‐FLAG M2 affinity Gel | Sigma‐Aldrich | Cat# A2220 |
| DSS (disuccinimidyl suberate) | Invitrogen Thermo Fisher | Cat# A39267 |
| Software | ||
| GraphPad Prism 8.0 | GraphPad, San Diego, CA | https://www.graphpad.com/scientific‐software/prism/ |
| ImageJ | National Institues of Health | https://imagej.nih.gov/nih‐image/ |
| Biorender | N/A | https://biorender.com/ |
| Ubpred | N/A | http://ubpred.org/ |
| Other | ||
| LDH‐Glo™ Cytotoxicity Assay | Promega | Cat# J2380 |
| Caspase‐Glo® 1 Inflammasome Assay | Promega | Cat# G9951l |
| Lipofectamine 2000 | Invitrogen | Cat# 11668‐019 |
| ELISA MAX™ Deluxe Set Mouse IL‐1β | Biolegend | Cat# 432616 |
| Mouse Caspase‐1 ELISA Kit | Novus biologicals | Cat# NBP2‐75014 |
| BD OptEIA™ Mouse TNF (Mono/Mono) ELISA Set | BD OptEIA | Cat# 555268 |
| BD OptEIA™ Mouse IL‐6 ELISA Set | BD OptEIA | Cat# 555240 |
| IL‐1 beta Human Uncoated ELISA Kit | Invitrogen | Cat# 88‐7261‐22 |
| Recombinant Mouse IL‐18 ELISA Set | MBL | Cat# B002‐5 |
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