Table 1.
Summary of the characterization results of the Nbs. With the name of the Nb, production yields in E. coli per liter, KD determined by SPR, and Tm.
| mTIGIT Nbs | Yield (mg/L) | KD (nM) | Tm (°C) |
|---|---|---|---|
| 16966 | 5.4 | 0.157 ± 0.001 | 68.08 ± 0.22 |
| 16972 | 4.3 | 0.152 ± 0.001 | 69.63 ± 0.10 |
| 16979 | 0.75 | 845.867 ± 8.070 | 58.82 ± 0.75 |
| 16988 | 1.7 | 0.104 ± 0.001 | 71.98 ± 0.48 |
| hTIGIT Nbs | Yield (mg/L) | KD (nM) | Tm (°C) |
|---|---|---|---|
| 16920 | 8.3 | 0.753 ± 0.002 | 63.15 ± 1.12 |
| 16925 | 3.8 | 1.974 ± 0.004 | 71.85 ± 0.55 |
| 17010 | 8.5 | 0.345 ± 0.001 | 65.87 ± 1.13 |
| 17018 | 15.1 | 0.470 ± 0.002 | 68.73 ± 1.33 |
| 17037 | 10.8 | 0.116 ± 0.001 | 59.30 ± 1.03 |
| ScFv Vibo | 9.7 | 0.996 ± 0.004 | 68.87 ± 0.33 |
KD, Equilibrium dissociation constant; Tm, Melting temperature; nM, nanomolar.