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. 1988 Aug;87(4):799–802. doi: 10.1104/pp.87.4.799

Localization of α-Amylase in the Apoplast of Pea (Pisum sativum L.) Stems 1

Eric P Beers 1, Stanley H Duke 1
PMCID: PMC1054847  PMID: 16666227

Abstract

Most of the activity of an α-amylase present in crude pea (Pisum sativum L. cv Laxton's Progress No. 9) leaf preparations cannot be found in isolated pea leaf protoplasts. The same extrachloroplastic α-amylase is present in pea stems, representing approximately 6% of total stem amylolytic activity and virtually all of the α-amylase activity. By a simple infiltration-extraction procedure, the majority (87%) of this α-amylase activity was recovered from the pea stem apoplast without significantly disrupting the symplastic component of the tissue. Only 3% of the β-amylase activity and less than 2% of other cellular marker enzymes were removed during infiltration-extraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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