Distribution of ESEQuant LF Reader measurements compared to consensus visual scoring of test lines in the measles IgM RDT.
Visual colour intensity of the Test Lines of valid RDTs was scored as follows: Zero (0): no visible line observed = Negative, One (1): uncertain reaction/ incomplete line = Indeterminate, Two (2): complete line of pale pink colouration = weak positive, Three (3): moderate pink line = medium positive, and Four (4): dark pink or red line = strong positive. The mean ESEQuant LF Reader Test Line measurement and 95% CI for each consensus visual Test Line scores are illustrated as horizontal black lines for the following visual scores: Score 0, 8.07 mV (95% CI, 3.56–12.59); Score 1, 63.1 mV; Score 2, 215.99 mV (95% CI, 180.16–251.81); Score 3, 471.98 mV (95% CI, 437.90–506.06), and Score 4, 701.26 mV (95% CI, 630.82–771.70). Upper and lower 95% sample population limits are illustrated as horizontal grey lines for each consensus visual Test Line score.