Table 5.

Information on the genomes and sequencing reads used in this article

Species	Genome size, estimated without sequencing (Mbp)	Genome size, estimated as the size of the assembly with the largest N50 among published assemblies (Mbp)	Sequencing technology	Sequence Read Archive identifiers of reads	N50 of reads (bp)	Approximate genome coverage by readsa
Trifolium pratense	636 [45], 474 [46], 418 [47], 557 [48]	423 [49]	PacBio HiFi	SRR15433789	20,082	50
Manihot esculenta	817 [50]	706 [39]	PacBio HiFi	ERR5485301	20,363	42
Heracleum sosnowskyi	1751 [48]	1629 [44]	PacBio HiFi	SRR23251371, SRR23251372	14,679	22
			Illumina Hi-C, paired-end	SRR23251383, SRR23251384	76	34
Myripristis murdjan	no estimates	835 [51]	PacBio CLR	ERR3449630, ERR3449634, ERR3449635, ERR3453872, ERR3453873, ERR3453874, ERR3453875, ERR3453876	24,966	50
			Illumina shotgun, paired-end b	ERR3655549	151	155
Adineta vaga	362c [52]	101c [53]	Oxford Nanopore	SRR13348928	38,562	50
			Illumina shotgun, paired-end b	SRR13348929	251	331
Mytilus coruscus	1858 [54]	1567 [55]	Oxford Nanopore	ERR3415816	24,641	50
			Illumina shotgun, paired-end b	ERR3431204	150	53

^aThe approximate genome coverage was calculated based on the following genome sizes: Trifolium pratense 450 Mbp, Manihot esculenta 750 Mbp, Heracleum sosnowskyi 1700 Mbp, Myripristis murdjan 850 Mbp, Adineta vaga 100 Mbp, Mytilis coruscus 1600 Mbp

^bUsed only for polishing, after the assembly

^cThe cause of the discrepancy between genome size estimates for Adineta vaga is unknown