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(A) Left: Structure of methionine (Met) and azidohomoalanine (AHA). Right: 18 biological samples were prepared as indicated and assigned to each channel of 18plex TMTpro reagent. (B) Workflow of AHA-TMT degradomics sample preparation. (C) Quality check western blot for AHA-containing proteome using streptavidin before immunoprecipitation.
