TABLE 1.
Groups of VanA elements recognized among 107 enterococci of diverse origins using 10 primer pairs derived from the sequence of the prototype element, Tn1546
| Group | PCR product with the following primer
paira:
|
No. of isolates | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| p1p2 | p3p4 | p5p6 | p7p8 | p9p10 | p11p12 | p13p14 | p15p16 | p17p18 | p19p1 | ||
| A | + | + | + | + | + | + | + | + | + | + | 20 |
| B | + | + | + | + | + | + | + | ++ | + | + | 2 |
| C | + | + | + | + | + | + | + | ++ | ++ | + | 1 |
| D | − | + | + | + | + | + | + | + | + | + | 7 |
| E | − | + | + | + | + | + | + | ++ | + | + | 3 |
| F | − | + | + | + | + | + | + | − | +/− | + | 4 |
| G | − | + | + | + | + | + | + | − | − | + | 7 |
| H | − | + | + | ++ | ++ | + | + | ++ | + | + | 23 |
| I | − | + | + | ++ | ++ | + | + | − | + | + | 1 |
| J | − | + | + | ++ | ++ | + | + | − | − | + | 1 |
| K | − | + | + | ++ | ++ | + | + | − | − | − | 2 |
| L | − | + | − | ++ | + | + | + | − | +/− | + | 1 |
| M | − | − | + | + | + | + | + | + | + | + | 2 |
| N | − | − | + | + | + | + | + | − | − | + | 2 |
| O | − | − | + | ++ | ++ | + | + | + | + | + | 1 |
| P | − | − | − | + | + | + | + | + | + | + | 1 |
| Q | − | − | − | + | + | + | + | ++ | + | + | 1 |
| R | − | − | − | ++ | ++ | + | + | ++ | + | + | 1 |
| S | − | − | − | − | + | + | + | ++ | + | + | 1 |
| T | − | − | − | − | ++ | + | + | ++ | + | + | 8 |
| U | − | − | − | − | − | + | + | ++ | + | + | 9 |
| V | − | − | − | − | − | + | + | ++ | ++ | + | 1 |
| W | − | − | − | − | − | + | + | − | +/− | + | 7 |
| X | − | − | − | − | − | + | + | − | − | + | 1 |
a
+, ++, +/−, amplicons the same size as, larger than, and smaller than those obtained for E. faecium BM4147 (containing Tn1546), respectively; −, absence of an amplicon with the particular pair of primers.