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Temperature-dependent transport of Atg9 vesicles. the lipid flippase Drs2 is required to deliver Atg9-positive vesicles to the PAS at low temperatures. Drs2 binds and stabilizes the multisubunit tethering complex TRAPPIII through the interaction between Trs85, the specific subunit of TRAPPIII, and the I(S/R)TTK motif present in the N-terminus of Drs2. At higher temperatures, Drs2 is dispensable for Atg9 transport.
