Figure 3.

Tethering PB1 aggregates to ATG16L1 or LC3 induces targeted autophagic degradation of PB1-streptavidin aggregates using streptavidin system. (A) The concept of streptavidin system. The streptavidin system consisting of the ligand SBP for streptavidin and the autophagy-targeting ligand LIR or ABP are expected to induce targeted degradation of PB1-streptavidin aggregates. (B) Immunoblot analysis of GFP-PB1-streptavidin and GFP-streptavidin upon streptavidin system expression. HEK293 cells stably expressing GFP-PB1-streptavidin and GFP-streptavidin were transiently transfected with streptavidin system SBP-LIR and SBP-ABP constructs. (C) Quantification of the protein levels of GFP-PB1-streptavidin and GFP-streptavidin as in (B). Data in (B) are presented as the mean ± SEM of three independent experiments. “ns”, no significant difference; **p < 0.01, Student’s t test.