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COS-7 cells were co-expressed with mCherry-SEC61-C18 and Myc-R3F. Immunofluorescence staining of R3F was performed with an anti-Myc antibody. Cell nuclei were revealed with DAPI staining. Scale bar: 10 μm. The rightmost column shows magnified images from the boxed region of each variant. Experiments were repeated three times.
