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. 2023 Jul 31;32(20):2966–2980. doi: 10.1093/hmg/ddad122

Figure 1.

Figure 1

ORF57 protects against oxidative stress in TDP-43𑀏NLS overexpressing SH-SY5Y cells. Experiments conducted in mCherry and ORF57 mCherry express ing SH-SY5Y TDP-43ΔNLS cells with and without SA stress. (A) Constructs for mCherry and ORF57::mCherry lentivirus. (B) Overexpression of ORF57 in SH-SY5Y cells by rt-qPCR. (C) Western blot for phosphorylated eIF2α and total eIF2α. (D) Quantification of C. (E) SUnSET of mCherry and ORF57 expressing TDP-43ΔNLS cells with SA stress. (F) Quantification of E. (G) Western blot of cleaved caspase-3 in TDP-43ΔNLS cells. (H) Quantification of G. (I) Western blot of cleaved caspase-3 in non-overexpressing SH-SY5Y cells. (J) Quantification of I.