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. 2023 Jul 31;32(20):2966–2980. doi: 10.1093/hmg/ddad122

Figure 2.

Figure 2

Stress granule assembly and TDP-43 aggregation dysregulated by ORF57.

(A) Immunocytochemistry in SH-SY5Y TDP-43ΔNLS. DAPI (405 nm). mCherry or ORF57::mCherry (594 nm). TDP-43ΔNLS::GFP (488 nm). G3BP1 (647 nm). Merge, overlay of all images. White arrows indicate overlap between ORF57 and TDP-43 aggregates. (B) Representation of small TDP-43ΔNLS aggregates 10–200 Aggrecount units. (C) Representation of large TDP-43ΔNLS aggregates 200–400 Aggrecount units. (D) Quantification of G3BP1 aggregates in each cell condition from A. (E) Mander’s coefficient of fraction of mCherry overlapping with TDP-43 signal. (F) Quantification of small TDP-43ΔNLS aggregates. (G) Quantification of large TDP-43ΔNLS aggregates. Two-way ANOVA with Bonferroni post hoc comparison, *P < 0.05, **P < 0.01, ***P < 0.0001.