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. Author manuscript; available in PMC: 2023 Oct 4.
Published in final edited form as: J Vis Exp. 2022 Sep 28;(187):10.3791/64556. doi: 10.3791/64556

Figure 2: The temporal patterns of astrocyte (GFAP) and microglia (IBA1) morphological changes after a CHI.

Figure 2:

(A) GFAP staining at low magnification shows the regional increase in staining seen in the cortex of the CHI group. The morphological appearance of the astrocytes is shown in the higher-magnification insets, which were taken from the middle brain sections and from the same regions of the cortex. (B) IBA1-positive staining in the cortex at 1 day, 7 days, and 2 months post-injury shows changes in microglia morphology in the neocortex after the CHI (n = 7-14, 50/50 male/female). The mice (CD-1/129 background) were 8 months old at the time of surgery. This figure has been adapted from 11 and reproduced with permission. Scale bar = 1 mm, 50 μm and 100 μm as indicated in the figure.