Fig. 3.

Contraction analysis of cMTs. (A) Brightfield images of cMTs from static control, 72 h flow monoculture and 72 h flow co-culture and their respective contraction plots upon electrical pacing at 1 Hz. Scale bar: 100 μm. Contraction amplitude is indicated as arbitrary unit (a.u.). (B–G) Contraction duration (B), time to peak (C), relaxation time (D), contraction duration at 50% of the amplitude (E), contraction amplitude (F) and triangulation indicated as adimensional (adim) (G) of cMTs paced at 1 Hz; n ≥ 11 from 4 independent batches; p < 0.0001, ANOVA results of Kruskal-Wallis test, followed by multiple comparisons. Data are shown as mean ± SD. (H) Percentage of cMTs following and not following pacing at 1 Hz; *p < 0.05, Chi-square test.