Table 6.
Effect of dietary supplementation of betaine (BT) and organic minerals (OMs) on broilers’ hepatic contents of MDA, SOD, and GPX under heat stress (HS).
| AT | BT, g/ton | OMs, g/ton | MDA, nmol/g | SOD, U/mg | GPX, U/mg |
|---|---|---|---|---|---|
| TN | 0 | 0 | 8.450 | 3.988 | 1.988 |
| 250 | 6.380 | 4.740 | 2.740 | ||
| 500 | 6.740 | 5.040 | 3.040 | ||
| 2000 | 0 | 7.436 | 4.760 | 2.760 | |
| 250 | 6.870 | 4.720 | 2.720 | ||
| 500 | 6.370 | 5.140 | 3.140 | ||
| HS | 0 | 0 | 10.61 | 2.200 | 1.060 |
| 250 | 7.870 | 3.420 | 1.420 | ||
| 500 | 7.556 | 3.740 | 1.740 | ||
| 2000 | 0 | 7.800 | 3.900 | 1.900 | |
| 250 | 7.126 | 3.520 | 1.520 | ||
| 500 | 7.280 | 3.520 | 1.520 | ||
| SEM | 0.233 | 0.129 | 0.115 | ||
| P value | |||||
| HS | 0.018 | >0.001 | >0.001 | ||
| BT | 0.050 | 0.015 | 0.109 | ||
| OMs | 0.003 | 0.007 | 0.096 | ||
| HS×BT | 0.235 | 0.454 | 0.891 | ||
| HS×OMs | 0.900 | 0.875 | 0.352 | ||
| BT×OMs | 0.156 | 0.003 | 0.064 | ||
| HS×BT×OMs | 0.625 | 0.285 | 0.830 |
Values provided represent means that have been deemed statistically significant at (P < 0.05). SEM = standard error of means; AT = ambient temperature; TN = thermoneutral temperature; MDA = malondialdehyde; SOD = superoxide dismutase; GPX = glutathione peroxidase. n = 10, 10 birds per treatment (2 birds per duplicate, 1 male and 1 female).