Fig. 3.

Proteomic characterization of JAR and JEG-3 isolated extracellular vesicles through liquid chromatography and mass spectrometry. A Gene ontology enrichment analysis performed on proteins identified in JAR and JEG-3 extracellular vesicles grouped based on biological processes. B Pathways involved in immune function identified in (A) in JAR and JEG-3 extracellular vesicles. C Immune system-related biological processes between JAR and JEG-3 isolated with adjusted p-values less than 0.05. D Western blot characterization of JAR extracellular vesicles and cell lysates for key exosome markers CD63, TSG101, and CD81, and non-extracellular vesicle marker nuclear antigen PCNA