Skip to main content
. 2023 Oct 4;14:6199. doi: 10.1038/s41467-023-41864-9

Fig. 2. Role of PEG in liquid-liquid phase separation of proteins in vitro.

Fig. 2

a Representative confocal microscopy images of selected NHS-Rhodamine labeled proteins [10% (v/v) labeled to unlabeled] (GG, β-lac, BSA, LYS and RNase A) in the presence of 10% PEG (w/v) (5% FITC-labeled PEG-5000 + 5% PEG-8000) showing LLPS with no PEG sequestration inside the condensates. All the samples were prepared under identical conditions using 20 mM sodium phosphate buffer (pH 7.4) and FITC-labeled PEG-5000 was added before the proteins undergo LLPS. The scale bar is 5 µm. b Representative fluorescence, DIC microscopy and DIC/fluorescence merged images of the selected NHS-Rhodamine labeled protein [1:10 (v/v) labeled to unlabeled protein] condensates at different conditions (Supplementary Table 3) in the absence of PEG-8000. Note the liquid condensate formation in the absence of PEG-8000 by other proteins is shown in Supplementary Fig. 8d. The scale bar is 5 μm. c Representative fluorescence microscopy images showing LLPS in cytoplasmic extract for selected NHS-Rhodamine labeled proteins (GG, β-lac, Chymo, BSA and CA). The scale bar is 5 μm. All the experiments were performed three independent times with similar observations.