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Scheme of two-step thyroid stimulating hormone (TSH) digital immunoassay (d-IA). ASD: assay specific diluent, BK: black dye, CNJ: conjugate, IR: immuno-reaction; Sub.: substrate buffer. Bottom left image: beads image, bottom right image: fluorescence image (0.5 μIU/mL of TSH). Scale bar, 100 μm.
