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. 2023 Sep 21;14:1209249. doi: 10.3389/fimmu.2023.1209249

Figure 1.

Figure 1

TRAIL does not majorly affect the viability of primary human macrophage subtypes. M0 macrophages were stimulated with 200 ng/ml TRAIL for 18 hours. For polarization, macrophages were pre-stimulated with TRAIL for 6 hours and then polarized into M1 (100 ng/ml LPS and 20 ng/ml IFNγ), M2a (20 ng/ml IL-4), or M2c (10 ng/ml IL-10) for 12 hours. Control groups were left unstimulated or stimulated with only M1, M2a, or M2c polarization factors for 12 hours. Cell viability was analyzed with Annexin V/7-AAD staining by flow cytometry. (A) Representative dot plots and (B) Bar graphs show the percentage of live macrophages (AnnexinV-7AAD-). Data shown are mean ± SEM pooled from two independent experiments (n=7). Statistical analyses were performed with a One-way ANOVA with Sidak’s multiple comparisons post-hoc test between untreated and TRAIL-treated macrophages, polarized and TRAIL-treated polarized macrophages, **P<0.01 and ***P<0.001.