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. 2023 Sep 21;14:1209249. doi: 10.3389/fimmu.2023.1209249

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Copyright © 2023 Gunalp, Helvaci, Oner, Bursalı, Conforte, Güner, Karakülah, Szegezdi and Sag

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TRAIL slightly increases phagocytosis of AML cells by macrophages. Macrophages were labelled with the cell tracker CFSE. Control and TRAIL-pre-stimulated (200 ng/ml, 6 h) macrophages were polarized into M1 with 100 ng/ml LPS+20 ng/ml IFNγ for 12 hours. After polarization, macrophages were washed and co-cultured with Tag-IT labelled U937 cells for 72 hours. Tag-IT Violet⁺ AML cells within live macrophages (CFSE⁺ Sytox Red^-) were analyzed by flow cytometry. (A) Representative dot plots with a gating strategy and (B) Bar graphs show the percentage of double positive live macrophages (CFSE⁺ Sytox Red^- Tag-IT Violet⁺). Data shown as symbols and lines pooled from four independent experiments (n= 7). Statistical analyses were performed with Wilcoxon matched-pairs signed-rank test between control and TRAIL-treated groups, *P<0.05.