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. 2023 Sep 21;14:1209249. doi: 10.3389/fimmu.2023.1209249

Figure 6.

Figure 6

TRAIL increases the expression of M1 markers in primary human M2c macrophages at the protein level. Macrophages were pre-stimulated with 200 ng/ml TRAIL for 2 hours and then polarized into M2c with 10 ng/ml IL-10 for 6 hours for the analyses of the CXCL10 and CXCL1. For the remaining markers, Macrophages were pre-stimulated with TRAIL for 6 hours and then polarized with IL-10 for 12 hours. Control groups were left unstimulated or stimulated only with 10 ng/ml IL-10 for 6 or 12 hours, respectively. (A–D) The production of CXCL10, CXCL1, IDO, and CD38 was analyzed by flow cytometry, and the representative plots are included. (E) The production of CXCL11 was analyzed by ELISA. Data shown are mean ± SEM or median with interquartile range pooled from two or more independent experiments [(A) n=9, (B) n=11, (C) n=6, (D) n=8, (E) n= 10]. Statistical analyses were performed with a One-way ANOVA with Sidak’s multiple comparisons post-hoc test, or Friedman with Dunn’s multiple comparisons post-hoc test between untreated and M2c macrophages, M2c and TRAIL-treated M2c macrophages, *P<0.05, ***P<0.001.