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Flowchart of stool samples processed for microbial profiling using 16S ribosomal RNA (rRNA) sequencing. Identical criteria were applied to exclusions of samples from the Framingham Heart Study and the Osteoporotic Fractures in Men Study cohorts including participants who reported use of antibiotics within 30 days of collection, a history of colon surgery in the year prior to collection, and samples from individuals lacking covariate data.
