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. 2023 Sep 27;2023:5548112. doi: 10.1155/2023/5548112

Figure 2.

Figure 2

ADSC-Exos promotes the proliferation and migration of DPCs and enhances hair inducibility. (a) Uptake of PKH67 fluorescently labeled ADSC-Exos by dermal papilla cells. Blue fluorescence: DAPI-stained nuclei; green fluorescence: PKH67-labeled ADSC-Exos, scale bar: 100 μm. (b) CCK-8 absorbance values of DPCs treated with different concentrations of ADSC-Exos at 24, 48, and 72 hr. (c) Scratch experimental plots of ADSC-Exos treated DPCs, scale bar: 100 μm. (d) Quantification of scratch area in Figure 4(b). (e) Graph of versican, ALP protein bands. (f) Relative quantitative graph of protein bands with grayscale values. (g) qPCR result of VEGF, HGF, BMP2, ALP, KGF, DKK-1, and IGF-1. The expression level of target genes was standardized to that of GAPDH. (h) Expression of HGF and IGF-1 detected by ELISA in the culture supernatant of DPCs; N = 3,  ∗P < 0.05,  ∗∗P < 0.01,  ∗∗∗P < 0.001, and #P < 0.05, 10 μg/mL compared with 20 μg/mL.