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. 2023 Jul 26;114(10):3957–3971. doi: 10.1111/cas.15912

FIGURE 3.

FIGURE 3

LOXL2 upregulates stemness marker protein expression and promotes spheroid formation. (A–C) Western blot analysis of Oct4, Sox2, and Nanog in TW2.6/LOXL2 (A), FaDu/LOXL2 (B), and SAS sphere cells (C) receiving two individual LOXL2 siRNAs (SAS‐sp/siLOXL2). (D–F) The sphere‐forming ability of TW2.6/LOXL2 (D), FaDu/LOXL2 (E), and SAS‐sp/siLOXL2 (F) cells. (G,H) Western blot analysis of Oct4, Sox2, and Nanog in TW2.6/LOXL2 (G) and FaDu/LOXL2 (H) cells transfected with two individual Snail siRNAs. (I,J) The sphere‐forming ability of TW2.6/LOXL2 (I) and FaDu/LOXL2 (J) cells transfected with two individual Snail siRNAs. (K,L) LOXL2 inhibitor (LOXL2‐IN‐1) did not affect the sphere‐forming ability of TW2.6/LOXL2 (K) and FaDu/LOXL2 cells (L). ****p < 0.0001. LOXL2, lysyl oxidase‐like protein 2.