Red dots indicated by yellow arrows represent the physical interaction among listed proteins in used cell lines. (A) PLA analysis of YAP1 in MUC13 knockdown cells (SW620+shMUC13), leading to decreased nuclear localization of YAP1 compared with the ShCtrl cells (scale bar, 10 μm). (B) PLA analysis of MUC13 and YAP1 in MUC13 knockdown cells (SW620+shMUC13) showed increased localization to the perinucleus compared with vector control (scale bar, 10 μm). (C) Confocal images show higher YAP1/β-catenin survival complexes in MUC13 overexpression (SW480+MUC13) cells compared to vector only. Most of the YAP1/β-catenin complexes are translocated to the nucleus (scale bar, 50 μm). (D) Knockdown of MUC13 prevented the nuclear translocation of the YAP1/β-catenin complex as it was predominantly localized outside the nuclear envelope in SW620+shMUC13 cells (scale bar, 20 μm). The images depict at least three independent experiments.