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. 2023 Oct 4;6(12):e202301975. doi: 10.26508/lsa.202301975

Figure S3. Influence of MUC13 on proteome and YAP1 phosporelation/subcellular localization.

Figure S3.

(A) (i, ii, iii) Key proteins involved in anchorage-independent survival. Proteomic analysis (iTRAQ/P.M.T.) of anoikis-induced cells (SW480 and SW620) at 36 h. Fold changes of the key proteins are shown (i). Immunoblot analysis of YAP1 protein to validate the result of proteomic analysis using isogenic cells SW480 and SW620 (ii), RTPCR analysis of YAP1 mRNA to show YAP1 mRNA expression precedes the translation event (iii). Immunoblot analysis of BIRC5 and SOX2 protein to validate the result of proteomic analysis (iv). (B) (i, ii) Immunoblot analysis of pYAP1 (Y357) in isogenic cell lines SW480 and SW620, and MUC13 overexpression SW480+Vec and SW480+MUC13. (C) Expanded panels of IF analysis of YAP1 in SW480+Vec and SW480+MUC13 from Fig 4E (scale bar, 50 μm). (D) Expanded panels of PLA analysis of MUC13_YAP1 in SW480+Vec and SW480+MCU13 form Fig 4F (scale bar, 50 μm).