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. 2023 Oct 4;6(12):e202301975. doi: 10.26508/lsa.202301975

Figure S5. MUC13 facilitates nuclear translocation of downstream oncol YAP1/β-catenigeneicn survival complex PLA was performed on pelleted, cryofixed, cryosection cells using the indicated anti-mouse (MAb) and anti-rabbit (PAb) antibodies and PLA probes.

Figure S5.

(A) β-catenin interaction promotes and (A) PLA analysis of MUC13 and β-catenin in MUC13 overexpressing cells (SW480+MUC13) show higher proximity of these two proteins in the nucleus compared with vector control. The images depict at least three independent experiments (scale bar, 50 μm). (B, C) qT–PCR analysis to determine the effect of MUC13 on YAP1/β-catenin survival complex downstream targets genes (C-Myc and Axin 2) in low and high MUC13-expressing isogenic SW480 and SW620, MUC13 overexpression SW480+MUC13 and SW480+Vector, and MUC13 knockdown SW620+shVector and SW620+shMUC13 in (B, C), respectively. YAP1 is required for MUC13-mediated cell survival, migration, and invasion. (D) PI-based cell cycle analysis shows knockdown of YAP1 in MUC13 overexpressing cells (SW480_MUC13+shYAP1) increases the sub-G0 population at different time points, indicating the requirement of YAP1 for MUC13-mediated survival function. (E) (i, ii) Invasion of knockdown of YAP1 in MUC13 overexpression cell lines and average quantification of the number of cells invaded (i) (scale bar, 50 μm). Migration of knockdown of YAP1 in MUC13 overexpression cell lines and average quantification of the number of cells invaded (ii) (scale bar, 50 μm). Unpaired t test, n = 4. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 denote significant differences.