Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 17;34(10):ar97. doi: 10.1091/mbc.E23-02-0063

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Sherwin et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License.

PMC Copyright notice

FIGURE 3: — Fin1-PP1 promotes CPC translocation in cells with inactive Cdc14. (A) Premature KT localization of Fin1-PP1 promotes Ipl1 translocation to the spindle in cdc14-2 mutants. cdc14-2 IPL1-3GFP TUB1-mCherry cells (4052-9-2) containing either FIN1 (pMB6) or fin1-5A (pMB7) plasmids were grown to log phase in synthetic dropout medium at 25°C then shifted to 37°C for 2 h to inactivate Cdc14. Colocalization of Ipl1 with spindle (Tub1-mCherry) was quantified. Yellow arrows represent Ipl1 spindle localization in the cell outlined with white-dotted line. Scale bar, 5 μm. The pictures are representative of three experimental repeats (N = 3) where 100 cells were counted for each experiment. The bars represent mean values ± SD. p values were obtained by using a two-tailed unpaired t test, with asterisks indicating *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. (B) fin1-5A restores Slk19 midzone localization in cdc14-2 cells. cdc14-2 SLK19-GFP TUB1-mCherry cells (4210-1-2) containing either FIN1 (pMB6) or phospho-deficient fin1-5A (pMB7) plasmids were grown to log-phase in YPD at 25°C then shifted to 37°C for 2 h to inactivate Cdc14. The percentage of cells with Slk19 midzone localization was quantified. The guide for counting midzone localization is shown in the schematic. Representative cells are outlined in white, with yellow arrows representing Slk19 midzone localization. Scale bar, 5 μm. The pictures are representative of three experimental repeats (N = 3) where 100 cells were counted for each experiment. The bars represent mean values ± SD. p values were obtained by using a two-tailed unpaired t test, with asterisks indicating *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. (C) fin1-5A does not restore Ase1 midzone localization in cdc14-2 cells. cdc14-2 ASE1-GFP TUB1-mCherry cells (4209-1-1) containing either FIN1 (pMB6) or fin1-5A (pMB7) plasmids were subjected to the same experiment as in panel B. The percentage of Ase1 midzone localization was quantified. The guide for counting midzone localization is shown in the schematic in panel B. Yellow arrows represent Ase1 midzone localization in the cell outlined with white dotted line. Scale bar, 5 μm. The pictures are representative of three experimental repeats. The statistical analysis was performed as described above.