Table 1.

Examples of morphometric descriptor (MD) values found in the literature

MD parameters	Value	Vessel type	Organ	Mouse strain	Vessel staining & Optical clearing (OC) methods	Imaging method	Software used	Reference
Density
Volumec (%)	34	Cap	LV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	19	Cap	S	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	28	Cap	RV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	31	Cap	LV	B6J	Lectin IV – iDisco+	CM	ImageJ	Nicolas and Roux19
	12	HEVs	PLNs	B6J	MECA-79 AB – BABB	LSM	Imaris	Jeucken et al.14
Volume (μm3)	1.6 x 105	Cap	IPIs	NOD	Lectin IV – No OC	CM	Imaris	El-Gohary et al.13
Surface (μm2)	4 x 10−5	BVs	Retina	B6J	CD31 AB – EyeCi	LSM	Imaris	Henning et al.3
Segmentsd	600,000	Cap	LV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	400,000	Cap	S	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	390,000	Cap	RV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	4,300,000	Cap	LV	B6J	Lectin IV – iDisco+	CM	ImageJ	Nicolas and Roux19
Segmentse	90	Cap	IPIs	NOD	Lectin IV – No OC	CM	Imaris	El-Gohary et al.13
Nodesd	290,000	Cap	LV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	170,000	Cap	S	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	190,000	Cap	RV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	2,000,000	Cap	LV	B6J	Lectin IV – iDisco+	CM	ImageJ	Nicolas and Roux19
Nodese	100	HEVs	PLNs	B6J	MECA-79 AB - BABB	LSM	Imaris	Jeucken et al.14
	55	Cap	IPIs	NOD	Lectin IV – No OC	CM	Imaris	El-Gohary et al.13
	190	BVs	Retina	B6J	CD31 AB - EyeCi	LSM	Imaris	Henning et al.3
Total length (μm)	13,000,000	Cap	LV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	11,500,000	Cap	S	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	7,110,000	Cap	RV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	27,000,000	Cap	LV	B6J	Lectin IV – iDisco+	CM	ImageJ	Nicolas and Roux19
	1,000,000	HEVs	PLNs	B6J	MECA-79 AB - BABB	LSM	Imaris	Jeucken et al.14
	3,000	Cap	IPIs	NOD	Lectin IV – No OC	CM	Imaris	El-Gohary et al.13
	23,000	BVs	Retina	B6J	CD31 AB - EyeCi	LSM	Imaris	Henning et al.3
	1,600,000	BVs	Ovary	B6J	RG - CLARITY	LSM	Imaris	Oren et al.5
Connectivity
Nodes/Segments	40,000	BVs	Ovary	B6J	RG – CLARITY	LSM	Imaris	Oren et al.5
	48%	Cap	LV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	43%	Cap	S	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	49%	Cap	RV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	46%	Cap	LV	B6J	Lectin IV – iDisco+	CM	ImageJ	Nicolas and Roux19
Architecture
Fractal Dimension	2.46	Cap	LV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	2.32	Cap	S	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	2.33	Cap	RV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	2.53	Cap	LV	B6J	Lectin IV – iDisco+	CM	ImageJ	Nicolas and Roux19
Diametera (μm)	4.8	Cap	LV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	3.8	Cap	S	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	5.1	Cap	RV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	3.1	Cap	LV	B6J	Lectin IV – iDisco+	CM	ImageJ	Nicolas and Roux19
	6.4	BVs	Retina	B6J	CD31 AB - EyeCi	LSM	Imaris	Henning et al.3
Lengthb (μm)	14.3	BVs	Ovary	B6J	RG – CLARITY	LSM	Imaris	Oren et al.5
	12.3	Cap	LV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	12	Cap	S	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	12.9	Cap	RV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
Straightness	0.9	BVs	Ovary	B6J	RG – CLARITY	LSM	Imaris	Oren et al.5
Tortuosityb	1.2	Cap	LV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	1.2	Cap	S	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19
	1.3	Cap	RV	B6J	Lectin IV – iDisco+	LSM	ImageJ	Nicolas and Roux19

OC, optical clearing; LV, cardiac left ventricle; RV, cardiac right ventricle; S, cardiac interventricular septum; PLNs, pair lymph nodes (brachial and inguinal); Cap, capillary; BVs, blood vessels; HEVs, high endothelial venules; B6J, C57BL6/J; IPIs, intrapancreatic islets; IV, intravenous injection; AB, antibody; RG, Vecad^cre x tdTomato^{flox/stop/flox} reporter gene; LSM, light-sheet microscopy; CM, confocal microscopy.

All MDs were obtained on wild type mice. Parameter values are given as rounded mean.

^aParameter value is the mean obtained by the frequency distribution after a non-linear regression of the date by a Gaussian curve.

^bParameter values are given as rounded median.

^cNormalized per mm³ of the tissue studied.

^dNumber per mm³ the tissue studied.

^eNumber per studied zone.