Skip to main content
. 2023 Sep 16;26(10):107960. doi: 10.1016/j.isci.2023.107960

Figure 2.

Figure 2

SQST-1 regulators include proteins that bind lipid droplets and that aggregate with age

(A) Pathway enrichment of genes that regulate SQST-1 levels using WormCat.44

(B) SQST-1:RFP accumulates after autophagy is inhibited by lgg-1 silencing for 3 days during adulthood at 25°C. Representative confocal microscopy image of the distal intestine of animals expressing both SQST-1:RFP and the lipid droplet-resident protein DHS-3 fused to GFP, subjected to lgg-1 silencing for 3 days during adulthood at 25°C. The co-localization between DHS-3:GFP and SQST-1:GFP is quantified.

(C) Overlap of SQST-1 regulators with age-dependent aggregating proteins2 and lipid droplet-associated proteins.40 Bolded genes were selected as representative.

(D) Representative images of gene silencing of a selected set of overlapping SQST-1 regulators during adulthood for 3 days at 25°C in animals over-expressing SQST-1:RFP. Fluorescence and tissue distribution quantified ±SD ANOVA ∗p < 0.05,∗∗∗∗p < 0.001. Details about SQST-1 regulators are available in Tables S1 and S2.