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. 2023 Sep 22;28(5):211. doi: 10.3892/mmr.2023.13098

Figure 6.

Figure 6.

The macrophages were divided into five groups: CTRL group; LPS group, macrophages were treated with 200 ng/ml LPS for 24 h; KA/LPS group, macrophages were treated with 100 µM KA for 0.5 h followed by 200 ng/ml LPS for 24 h; LPS/KA/ML385 group, macrophages were treated with 5 µM ML385 for 0.5 h, 100 µM KA for 0.5 h, followed by 200 ng/ml LPS for 24 h; and ML385 alone group, macrophages were treated with 5 µM ML385 for 24 h. (A and B) Hoechst-PI staining. (C) CCK-8 assay. (D) LDH assay. (E and F) The protein expression levels of NLRP3, Caspase1, and GSDMD in macrophages. The levels of inflammatory cytokines in the cell supernatant were detected. (G) IL-1β, (H) IL-18, and (I) TNF-α. ***P<0.001 vs. CTRL group; ###P<0.001 vs. LPS group; &&&P<0.001 vs. LPS/KA group. n=3 per group. KA, Kynurenic acid; LPS, lipopolysaccharide; PI, propidium iodide; LDH, lactate dehydrogenase; NLRP3, NOD-like receptor protein 3; GSDMD, Gasdermin-D.