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. 2023 Oct 5;24:223. doi: 10.1186/s13059-023-03071-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Multiple alignment of protein sequences encoded in barley pangene cluster Horvu_MOREX_1H01G011400, produced with Clustalx. This cluster contains isoforms from 13 gene models, but none from genotype OUN333. The last sequence is encoded by a CDS sequence lifted-over from cultivar HOR3081 on the genome of OUN3, spanning 3 exons (exon boundaries are marked with asterisks. B, Patch GFF file with the coordinates of the exons lifted-over from gene model Horvu_3081_1H01G015200. The underlying CDS nucleotide sequence was aligned with 411 matches, no indels and no mismatches with check_evidence.pl -f